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GrainGenes Probe Report: CDO949

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Probe
CDO949  [ Marker Report ]
Locus
Xcdo949-4D
[ Show all 9 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439311
BE439344
CDO949_WHE05E05.s1
Polymorphism
CDO949 DraI
CDO949 XbaI
CDO949 EcoRI
CDO949 EcoRV
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO949 KO autoradiogram
CDO949 SO autoradiogram
Data Source
Sorrells, Mark E.96.07