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GrainGenes Probe Report: CFA2019

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Probe
CFA2019  [ Marker Report ]
Locus
Xcfa2019-7A
Sr22
cfa2019
Xcfa2019
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)14
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' GACGAGCTAACTGCAGACCC 3'
Reverse 5' CTCAATCCTGATGCGGAGAT 3'
SSR size
217
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu
Data Source
Sourdille, Pierre