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GrainGenes Probe Report: CFA2153

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Probe
CFA2153  [ Marker Report ]
Locus
Xcfa2153-1A
Xcfa2153-1A.1
Xcfa2153-1A.2
Xcfa2153
H9/H10/H11
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = A
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CT)27
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TTGTGCATGATGGCTTCAAT 3'
Reverse 5' CCAATCCTAATGATCCGCTG 3'
SSR size
200
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A
Source Species
Triticum urartu
Data Source
Sourdille, Pierre