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GrainGenes Probe Report: CFD21

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
CFD21  [ Marker Report ]
Locus
Xcfd21-1D
[ Show all 15 ]
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = C
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)21
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' CCTCCATGTAGGCGGAAATA 3'
Reverse 5' TGTGTCCCATTCACTAACCG 3'
SSR size
281
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7D/1D
Source Species
Aegilops tauschii
Data Source
Sourdille, Pierre

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