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GrainGenes Probe Report: CFD23

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Probe
CFD23  [ Marker Report ]
Locus
Xcfd23-4D
Xcfd23
Lr67/Yr46/Sr55/Pm46/Ltn3
cfd23
cfd023
Reference
ReferenceHiebert CW et al. (2010) An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67) Theoretical and Applied Genetics 121:1083-1091.
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
ReferenceHiebert CW MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Leaf Rust Resistance. Lr67 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (AGT)23(GCA)3(GTA)8
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' TAGCAGTAGCAGCAGCAGGA 3'
Reverse 5' GCAAGGAAGAGTGTTCAGCC 3'
SSR size
187
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4D
Source Species
Aegilops tauschii
Data Source
Sourdille, Pierre

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