GrainGenes Probe Report: CFD23

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Probe

CFD23 [ Marker Report ]

Locus

Xcfd23-4D

Xcfd23

Lr67/Yr46/Sr55/Pm46/Ltn3

cfd23

cfd023

Reference

Hiebert CW et al. (2010) An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67) Theoretical and Applied Genetics 121:1083-1091.

Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.

Hiebert CW MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Leaf Rust Resistance. Lr67 MAS Wheat. Marker Assisted Selection in Wheat.

General Remarks

Quality Score = B

Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)

Amplifies motif: (AGT)23(GCA)3(GTA)8

Regularity of motif is imperfect

Type

PCR

SSR

PCR primers

Forward 5' TAGCAGTAGCAGCAGCAGGA 3'
Reverse 5' GCAAGGAAGAGTGTTCAGCC 3'

SSR size

187

Amplification Conditions

5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees

Linkage Group

Source Species

Aegilops tauschii

Data Source

Sourdille, Pierre


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