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GrainGenes Probe Report: EBmac560

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Probe
EBmac560  [ Marker Report ]
Locus
EBmac0560a
[ Show all 7 ]
Synonym
Other nameEBmac0560
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)7
Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type
PCR
SSR
PCR primers
5' CATATGTGTGCAAGTGTGC 3'
5' AGATCCATGAGGTTGTGC 3'
SSR size
171
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare