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GrainGenes Probe Report: EBmac615

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Probe
EBmac615  [ Marker Report ]
Locus
EBmac0615
EBmac615
Synonym
Other nameEBmac0615
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (TG)5CG(TG)3,(TG)10
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Diversity Index (DI) = 0.23
Type
PCR
SSR
PCR primers
5' AATTGGTTCGAGTCATAGCT 3'
5' CTAGTGGGTGTATGCAAGTG 3'
SSR size
173
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare