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GrainGenes Probe Report: EBmac824

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
EBmac824  [ Marker Report ]
Locus
EBmac0824
EBmac824
Synonym
Other nameEBmac0824
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (TG)4, (TG)4, (TG)4, (GT)4, (GT)4
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.43
Type
PCR
SSR
PCR primers
5' GCAAGCTTCCTAAATCCTTA 3'
5' TGCAGACAGTTTTTCATATACA 3'
SSR size
308
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species
Hordeum vulgare

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