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GrainGenes Probe Report: GBS546

Probe
GBS546  [ Marker Report ]
Locus
GBS0546
GBS0546-1H
Reference
ReferenceStein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks
Clone HF19G17r
PCR primers
AACTCTCGTGTCAATGCCGA
CAACGTATTGCAAGGTCGCA
Amplification Conditions
5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BU988993
DNA Library
IPK-HF
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
Data Source
Stein, Nils2006.10