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GrainGenes Probe Report: HvID

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Probe
HvID  [ Marker Report ]
Locus
HvID
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)16(AT)10
Quality = 2: Single product, relatively strong stutter bands
Null in H. spontaneum Canada Park.
Type
PCR
SSR
PCR primers
5' GACATTTTTTATAAATTAAGAGCG 3'
5' ATTAACAATCTGCATTAATTGTG 3'
GACATTTTTTATAAATTAAGAGCG
ATTAACAATCTGCATTAATTGTG
SSR size
182
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Sequence
D10057
Source Species
Hordeum vulgare

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