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GrainGenes Probe Report: ISU1410

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Probe
ISU1410  [ Marker Report ]
Locus
Xisu1410b
Xisu1410d
Xisu1410a
ISU1410
Xisu1410e
Reference
ReferenceRayapati PJ et al. (1994) A linkage map of diploid Avena based on RFLP loci and a locus conferring resistance to nine isolates of Puccinia coronata var. 'avenae' Theoretical and Applied Genetics 89:831-837.
General Remarks
Oat Uni-ZapXR
Type
cDNA
Amplification Conditions
94 C/1 min, 50 C/40 sec, 72 C/1 min, 34 cycles, 72 C/10 min
Polymorphism
ISU1410 KpnI
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Lang CIav9257
Source Tissue
etiolated root tissue
Roots of 8-day-old etiolated oat seedlings
Insert Size
0.5
Clone Vector
pBluSK
Unizap-XR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13 forward and reverse
Sk and T7
Clone Location
Lee, Michael
Clone Authority
Lee, Michael
Image
Crown rust resistance markers
Crown rust genes, diploid vs. hexaploid oat
Data Source
Bush, Arla L.95.05