Query (optional)   in Class  

GrainGenes Probe Report: ISU1774

Probe
ISU1774  [ Marker Report ]
Locus
Xisu1774
[ Show all 9 ]
Reference
ReferenceRayapati PJ et al. (1994) A linkage map of diploid Avena based on RFLP loci and a locus conferring resistance to nine isolates of Puccinia coronata var. 'avenae' Theoretical and Applied Genetics 89:831-837.
General Remarks
Oat Uni-ZapXR
Type
cDNA
Amplification Conditions
94 C/1 min, 50 C/40 sec, 72 C/1 min, 34 cycles, 72 C/10 min
Polymorphism
ISU1774 EcoRI
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Lang CIav9257
Source Tissue
Root
etiolated root tissue
Roots of 8-day-old etiolated oat seedlings
Insert Size
0.8
Clone Vector
pBluSK
Unizap-XR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13 forward and reverse
Sk and T7
Clone Location
GrainGenes Probe Repository
Lee, Michael
Clone Authority
Lee, Michael
Image
Crown rust resistance markers
Crown rust genes, diploid vs. hexaploid oat
Data Source
Bush, Arla L.95.05