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GrainGenes Probe Report: PSR109

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Probe
PSR109  [ Marker Report ]
Locus
Xpsr109(Rbcs)-2A
[ Show all 22 ]
Related Probe
PSR133
[ Show all 17 ]
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
General Remarks
Polymorphism scores are given as the percent polymorphic pairwise comparisons, followed by the number of alleles found and number of varieties tested in parentheses.
Type
cDNA
Sequence
PSR109
Copy Number
3
Background
2
Cross hybridizes to
Secale cerealegood
Hordeum vulgaregood
Polymorphism
PSR109 H EcoRI80.3 (4, 12)
[ Show all 16 ]
Gel
PSR109 Gale
Linkage Group
2AS
[ Show all 9 ]
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
Source Tissue
Leaf
Insert Size
0.65
Clone Vector
pUC18
Vector Enzyme
SmaI
Excision Enzyme
HindIII
EcoRI
Vector PCR primers
5'-d(GTTTTCCCAGTCACGAC)-3'
5'-d(CAGGAAACAGCTATGAC)-3'
Clone Location
Gale, Michael D.
Devos, Katrien M.
Clone Authority
Chao, Shiaoman
Devos, Katrien M.
Data Source
Gale, Michael D.97.09