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GrainGenes Probe Report: PSR144

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Probe
PSR144  [ Marker Report ]
Locus
Xpsr144-4A
[ Show all 11 ]
Related Probe
PSR111
PSR198
PSR200
PSR201
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
General Remarks
Polymorphism scores are given as the percent polymorphic pairwise comparisons, followed by the number of alleles found and number of varieties tested in parentheses.
Type
cDNA
Sequence
PSR144 Forward
PSR144 Reverse
Copy Number
1
Background
0
Cross hybridizes to
Secale cerealegood
Hordeum vulgaregood
Polymorphism
PSR144 H EcoRI20.0 (2, 10)
[ Show all 17 ]
Gel
PSR144 Gale
Linkage Group
4AL
[ Show all 6 ]
Insert Enzyme
SmaI
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
Source Tissue
Leaf
Insert Size
1.2
Clone Vector
pUC18
Vector Enzyme
SmaI
Excision Enzyme
HindIII
EcoRI
Vector PCR primers
5'-d(GTTTTCCCAGTCACGAC)-3'
5'-d(CAGGAAACAGCTATGAC)-3'
Clone Location
Gale, Michael D.
Clone Authority
Chao, Shiaoman
Data Source
Gale, Michael D.97.09
ATMI92.09