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GrainGenes Probe Report: PSR169

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Probe
PSR169  [ Marker Report ]
Locus
Xpsr169-7A
Xpsr169-7B
Xpsr169-7D
Xpsr169(Caa)
Xpsr169
Related Probe
PSR56
PSR117
PSR124
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceChao S et al. (1989) RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theoretical and Applied Genetics 78:495-504.
General Remarks
Polymorphism scores are given as the percent polymorphic pairwise comparisons, followed by the number of alleles found and number of varieties tested in parentheses.
Type
cDNA
Sequence
PSR169 Forward
PSR169 Reverse
Copy Number
1
Background
3
Cross hybridizes to
Secale cerealegood
Hordeum vulgaregood
Polymorphism
PSR169 7A HindIII7A 0.0 (1, 26)
[ Show all 9 ]
Gel
PSR169 Gale
Linkage Group
7AL
7BL
7DL
7H
7RL
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
Source Tissue
Leaf
Insert Size
0.9
Clone Vector
pUC18
Vector Enzyme
SmaI
Excision Enzyme
HindIII
EcoRI
Vector PCR primers
5'-d(GTTTTCCCAGTCACGAC)-3'
5'-d(CAGGAAACAGCTATGAC)-3'
Clone Location
Gale, Michael D.
Clone Authority
Chao, Shiaoman
Data Source
Gale, Michael D.97.09