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GrainGenes Probe Report: SNF-A2

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Probe
SNF-A2
Locus
Yr48
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
ReferenceHegarty J et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr48 MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers
SNF-A2-F 5'- TCC GTC TCC ATC ATT CAA CA
SNF-A2-R 5'- GTG TTG CGC AAG TTT GTG AC
Amplification Conditions
Denaturing step: 94C, 3 min. Amplification step (40 cycles): 95C, 30 sec; 58C, 30 sec; 72C, 30 sec. Extensions step: 72C, 5 min