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GrainGenes Probe Report: WMC113

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Probe
WMC113  [ Marker Report ]
Locus
Xwmc113-1B
Xwmc113-6D
Xwmc113c
Xwmc113x
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8f9
Donor: Flore, Verneuil
AGR177. Trimmed to insert 5' and to Rsa1 at 3'. (GT)18 36 to 71. Redesign primers if possible.
Type
PCR
SSR
PCR primers
Forward cAAgTTgAccATcgAcTcATAA at 8 length 22 bases, Tm 59
Reverse AAcTggAAgccAcgAcAAcATA at 73 length 22 bases, Tm 63
SSR size
87 bases expected, Chinese Spring
Amplification Conditions
51C anneal, smear;
Sequence
WMC113
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06