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GrainGenes Probe Report: WMC160

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Probe
WMC160  [ Marker Report ]
Locus
Xwmc160-5B
[ Show all 10 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11e6
Donor: Rogers, Monsanto
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 174 bases located on 5B
AGR204.45.SEQ. Trimmed to insert at both ends. (CA)24 60 to 107. Forawrd primer has weak homology to a seequence beyond reverse primer (does not involve 3' end of primer. True product is 169 bases (excluding terminal A), false priming may give product 226 bases.
Type
PCR
SSR
PCR primers
Forward cATggcTccAAgATAcAAAAAg at 6 length 22 bases, Tm 60
Reverse AggccTggATTcATgATAgATA at 153 length 22 bases, Tm 59
SSR size
169 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose;
Sequence
WMC160
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06