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GrainGenes Probe Report: WMC170

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Probe
WMC170  [ Marker Report ]
Locus
Xwmc170b-2A
Xwmc170a-2D
Xwmc170-2A
Xwmc170
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9h2
Donor: Bernard, Clermont-Ferrand
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 227 bases located on 2A
Trimmed to Rsa1 at both ends. Multiple inserts in this clone joined by linkers at 258-275. (CA)19 378 to 415. Primers are to second insert.
Type
PCR
SSR
PCR primers
Forward AcATccAcgTTTATgTTgTTgc at 331 length 22 bases, Tm 61
Reverse TTggTTgcTcAAcgTTTAcTTc at 539 length 22 bases, Tm 61
SSR size
230 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC170
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06