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GrainGenes Probe Report: WMC177

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC177  [ Marker Report ]
Locus
Xwmc177-2A
Xwmc177
wmc177-2A
wmc177
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9h4
Donor: Bernard, Clermont-Ferrand
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 190 bases located on 2A
Trimmed to insert at 5' end and to Rsa1 at 3' end. After first 40 bases is similar to 7c7(CA)21 37 to 78, (CGCA)10. Bases 81 to 101 show homology to 17e7 bases 273 to 293 (21 in longest run, maxScore = 21). Bases 37 to 70 show homology to 10h2 bases 68 to 101 (34 in longest run, maxScore = 26).
Type
PCR
SSR
PCR primers
Forward AgggcTcTcTTTAATTcTTgcT at 13 length 22 bases, Tm 59
Reverse ggTcTATcgTAATccAccTgTA at 175 length 22 bases, Tm 57
SSR size
184 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC177
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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