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GrainGenes Probe Report: WMC235

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Probe
WMC235  [ Marker Report ]
Locus
Xwmc235
Xwmc235-5B
wmc235-5B
wmc235
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5d1
Donor: Sharp, Camden
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 231 bases located on 5B
Trimmed to insert at 5' end and to Rsa1 at 3' end.(CA)25 178 to 227
Type
PCR
SSR
PCR primers
Forward AcTgTTccTATccgTgcAcTgg at 86 length 22 bases, Tm 65
Reverse gAggcAAAgTTcTggAggTcTg at 299 length 22 bases, Tm 64
SSR size
235 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC235
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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