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GrainGenes Probe Report: WMC25

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC25  [ Marker Report ]
Locus
Xwmc25b-2B
[ Show all 19 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2c9
Donor: Wang, Utah
(GT)26 193 to 244. >2 Polymorphic loci.
Type
PCR
SSR
PCR primers
Forward TcTggccAggATcAATATTAcT at 102 length 22 bases, Tm 59.0
Reverse TAAgATAcATAgATccAAcAcc at 246 length 22 bases, Tm 53.0
SSR size
166 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC25
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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