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GrainGenes Probe Report: WMC254

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC254  [ Marker Report ]
Locus
Xwmc254-1A
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4b2
Donor: Gupta, Meerut
No trimming done to sequence. (CA)28 31 to 86. Forward primer is not ideal.
Type
PCR
SSR
PCR primers
Forward AgTAATcTggTccTcTcTTcTTcT at 2 length 24 bases, Tm 58
Reverse AggTAATcTccgAgTgcAcTTcAT at 171 length 24 bases, Tm 64
SSR size
193 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC254
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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