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GrainGenes Probe Report: WMC273

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

WMC273  [ Marker Report ]
[ Show all 18 ]
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 3g8
Donor: Keller, Zurich
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 182 bases located on 7B, 5D, 199 bases located on 7D, 5D, 1A, 235 bases located on 7A, 5D
No trimming done to sequence. (GT)8 395 to 410, (GT)13 453 to 478.
PCR primers
Forward AgTTATgTATTcTcTcgAgccTg at 323 length 23 bases, Tm 58
Reverse ggTAAccAcTAgAgTATgTccTT at 479 length 22 bases, Tm 55
SSR size
179 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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