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GrainGenes Probe Report: WMC329

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Probe
WMC329  [ Marker Report ]
Locus
Xwmc329-1B
[ Show all 11 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17g10
Donor: Khairallah, Mexico
Trimmed to insert at both ends. Bases 78 to 107 show homology to 11b10 bases 167 to 196 (30 in longest run, maxScore = 55), zone is 78-255. Reverse primer has two mismatches to 11b10, forward primer is not within the zone of homology. (GT)21 68 to 109.
Type
PCR
SSR
PCR primers
Forward AcAAAggTgcATTcgTAgA at 16 length 19 bases, Tm 56
Reverse AAcAcgcATcAgTTTcAgT at 115 length 19 bases, Tm 56
SSR size
118 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide (beware of primer dimer).
Sequence
WMC329
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06