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GrainGenes Probe Report: WMC335

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC335  [ Marker Report ]
Locus
Xwmc335
Xwmc335-7B
wmc335
wmc335-7B
Xwmc335-6A
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12g3
Donor: Weibull, Svaloff Weibull
Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 11 to 30 show homology to 9f4 bases 305 to 324 (20 in longest run, maxScore = 20), zone is 1-45. (CA)5G(CA)12 88 to 113.
Type
PCR
SSR
PCR primers
Forward TgcggAgTAgTTcTTccccc at 36 length 20 bases, Tm 65
Reverse AcATcTTggTgAgATgcccT at 131 length 20 bases, Tm 61
SSR size
115 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC335
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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