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GrainGenes Probe Report: WMC382

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Probe
WMC382  [ Marker Report ]
Locus
Xwmc382-2A
[ Show all 15 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10f7.2
Donor: Jacquemin, Gembloux
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 159 bases located on 2A, 171 bases located on 2B, 222 bases located on 2A
Trimmed to insert at both ends. (CA)8 409 to 424, (GA)28 425 to 480, (GT)17 69 to 102.
There are two microsatellites, the primers here are for the second.
Type
PCR
SSR
PCR primers
Forward cATgAATggAggcAcTgAAAcA at 382 length 22 bases, Tm 66
Reverse ccTTccggTcgAcgcAAc at 572 length 18 bases, Tm 67
SSR size
217 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide - probably two polymorphic loci, 2 monomorphic loci.
Sequence
WMC382
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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