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GrainGenes Probe Report: WMC395

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC395  [ Marker Report ]
Locus
Xwmc395
wmc395
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10f12
Donor: Jacquemin, Gembloux
Ns in sequence may be Ts. Trimmed to Rsa1 at 5' end, 3' end not located. Bases 114 to 159 show homology to 7d11 bases 96 to 141 (46 in longest run, maxScore = 67). (CT)7 87 to 100, (CT)9 112 to 129.
Type
PCR
SSR
PCR primers
Forward TccAcAcAcATAgAcgcAAAgA at 21 length 22 bases, Tm 63
Reverse AcTgccAggAcTgTTgATgAAT at 176 length 22 bases, Tm 63
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, smear.
Sequence
WMC395
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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