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GrainGenes Probe Report: WMC44

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Probe
WMC44  [ Marker Report ]
Locus
Xwmc44-1B
[ Show all 6 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6e2
Donor: Dubcovsky, Buenos Aires
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 232 bases located on 1B
AGR61. Trimmed to Rsa1 at 3' end. (GT)35 341 to 410. 5 polymorphic loci.
Type
PCR
SSR
PCR primers
Forward ggTcTTcTgggcTTTgATccTg at 237 length 22 bases, Tm 66
Reverse TgTTgcTAgggAcccgTAgTgg at 457 length 22 bases, Tm 67
SSR size
242 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC44
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06