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GrainGenes Probe Report: WMC473

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Probe
WMC473  [ Marker Report ]
Locus
Xwmc473-4D
[ Show all 9 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e2
Donor: Jizeng, Beijing
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 111 bases located on 1D, 149 bases located on 4D
Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)27 40 to 93.
Type
PCR
SSR
PCR primers
Forward TcTgTTgcgcgAAAcAgAATAg at 6 length 22 bases, Tm 64
Reverse cccATTggAcAAcAcTTTcAcc at 122 length 22 bases, Tm 65
SSR size
138 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product. tested 51C anneal, no product.
Sequence
WMC473
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06