Query (optional)   in Class  

GrainGenes Probe Report: WMC487

[ Printable Version ]  [ Submit comment/correction ]

Probe
WMC487  [ Marker Report ]
Locus
Xwmc487-6B
wmc487-6B
wmc487
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25f7
Donor: Serasem
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 163 bases located on 6B
Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)20 120 to 159.
Type
PCR
SSR
PCR primers
Forward cAAATTTggccAccATTTTAcA at 53 length 22 bases, Tm 63
Reverse cggTTcAATccTTggATTTAcA at 191 length 22 bases, Tm 60
SSR size
160 bases expected, Chinese Spring
Amplification Conditions
tested 51C anneal, no product.
Sequence
WMC487
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06