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GrainGenes Probe Report: WMC489

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Probe
WMC489  [ Marker Report ]
Locus
Xwmc489a
[ Show all 24 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25f8
Donor: Serasem
Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 13 to 97 show homology to 7e6 bases 60 to 144 (85 in longest run, maxScore = 75). (CA)13 98 to 123. Bases 98 to 189 show homology to 26f11 bases 81 to 172 (92 in longest run, maxScore = 146). Duplicate with 26f11, 25f8 has priority.
Type
PCR
SSR
PCR primers
Forward cgAAggATTTgTgATgTgAgTA at 37 length 22 bases, Tm 59
Reverse ggAcAAcATcATAgAgAAggAA at 247 length 22 bases, Tm 59
SSR size
232 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (various bands 176-270 bases, complex)
Sequence
WMC489
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06