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GrainGenes Probe Report: WMC491

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

WMC491  [ Marker Report ]
[ Show all 8 ]
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25a9
Donor: Serasem
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 194 bases located on 4A
Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 257 to 269 (complement) show homology to 12d2 bases 27 to 39 (13 in longest run, maxScore = 33). Bases 112 to 131 show homology to 25c11 bases 73 to 92 (20 in longest run, maxScore = 46). (GT)28 151 to 206, (GT)5 251 to 260.
PCR primers
Forward ggTAAAAcTTcgTgTcccTTgc at 56 length 22 bases, Tm 64
Reverse TAgTTgcgAgTcggTAgTcTgc at 226 length 22 bases, Tm 64
SSR size
192 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (116-126 bases, 198-202 bases).
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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