Query (optional)   in Class  

GrainGenes Probe Report: WMC505

[ Printable Version ]  [ Submit comment/correction ]

Probe
WMC505  [ Marker Report ]
Locus
Xwmc505-3A
[ Show all 11 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 20g8
Donor: Byrne, Colorado State
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 115 bases located on 3A, 133 bases located on 3B, 90 bases located on 3D, 98 bases located on 3B
No trimming done to sequence at Agrogene, ends not located. (CA)31 55 to 116.
Type
PCR
SSR
PCR primers
Forward AggggAggAAAAccTTgTAATc at 28 length 22 bases, Tm 61
Reverse AcgAccTAcgTggTAgTTcTTg at 131 length 22 bases, Tm 60
SSR size
125 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (several loci) .
Sequence
WMC505
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06