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GrainGenes Probe Report: WMC511

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Probe
WMC511  [ Marker Report ]
Locus
Xwmc511a
Xwmc511-4B
wmc511
wmc511-4B
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 20g7
Donor: Byrne, Colorado State
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 4B, 130 bases located on 4B, 205 bases located on 4B
Trimmed to insert at 3' end, 5 ' end not located. (GT)7 148 to 161, (GT)4 172 to 179, also complex CGTG repeats. Related to 23b5, reverse primer has 4 mismatches to 23b5, forward primer has no match.
Type
PCR
SSR
PCR primers
Forward cgcAcTcgcATgATTTTccT at 75 length 20 bases, Tm 65
Reverse ATgcccggAAAcgAgAcTgT at 257 length 20 bases, Tm 66
SSR size
202 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC511
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06