Query (optional)   in Class  

GrainGenes Probe Report: WMC517

[ Printable Version ]  [ Submit comment/correction ]

Probe
WMC517  [ Marker Report ]
Locus
Xwmc517-7B
Xwmc517
wmc517-7B
wmc517
wmc517-4A
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22h1
Donor: Bright, Zeneca
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 186 bases located on 7B
Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 219 to 232 show homology to 7g4 bases 201 to 214 (14 in longest run, maxScore = 41). Reverse primer has 5 mismatches to 7g4, forward primer no apparent homology. (CA)16 36 to 67, (CA)6 113 to 124.
Type
PCR
SSR
PCR primers
Forward ATccTgAcgTTAcAcgcAcc at 5 length 20 bases, Tm 63
Reverse AccTggAAcAccAcgAcAAA at 173 length 20 bases, Tm 63
SSR size
188 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (180-200 bases)
Sequence
WMC517
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06