Query (optional)   in Class  

GrainGenes Probe Report: WMC522

[ Printable Version ]  [ Submit comment/correction ]

Probe
WMC522  [ Marker Report ]
Locus
Xwmc522-2A
Xwmc522
wmc522-2A
wmc522
wmc522-3D
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22h5
Donor: Bright, Zeneca
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 2A
Trimmed to insert at both ends. (CT)36 74 to 145, (TC)4 163 to 170.
Type
PCR
SSR
PCR primers
Forward AAAAATcTcAcgAgTcgggc at 5 length 20 bases, Tm 63
Reverse cccgAgcAggAgcTAcAAAT at 178 length 20 bases, Tm 63
SSR size
193 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (180-200 bases).
Sequence
WMC522
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06