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GrainGenes Probe Report: WMC59

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC59  [ Marker Report ]
Locus
Xwmc59-1A
[ Show all 9 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1e8
Donor: Waugh, SCRI
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 208 bases located on 1A
(CA)19 103 to 140. Bands a little weak. Barley - mapped 1HL tip Lina x Canada Park (R. Waugh et al) - at 55C anneal.
Type
PCR
SSR
PCR primers
Forward TcATTcgTTgcAgATAcAccAc at 74 length 22 bases, Tm 63
Reverse TcAATgcccTTgTTTcTgAccT at 249 length 22 bases, Tm 65
SSR size
197 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC59
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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