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GrainGenes Probe Report: WMC74

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Probe
WMC74  [ Marker Report ]
Locus
Xwmc74
[ Show all 9 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6A7
Donor: Devaux, Florimond Desprez
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 234 bases located on 5A, 256 bases located on 4D, 310 bases located on 4B, 5D
AGR97. Trimmed to Rsa1 at 3'.(GT)34 190 to 257. (4 polymorphic loci). Related to 1E4
Type
PCR
SSR
PCR primers
Forward AAcggcATTgAgcTcAccTTgg at 2 length 22 bases, Tm 69
Reverse TgcgTgAAggcAgcTcAATcgg at 285 length 22 bases, Tm 73
SSR size
305 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC74
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06