GrainGenes

GrainGenes Probe Report: WMS458

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMS458  [ Marker Report ]
Locus	Xgwm458-1D Xgwm458 gwm458-1D gwm458
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)13 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AATGGCAATTGGAAGACATAGC 3' 5' TTCGCAATGTTGATTTGGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS458
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Data Source	Roeder, Marion S. 98.09.15

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