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GrainGenes Probe Report: csSr2

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Probe
csSr2
Locus
Sr2
Reference
ReferenceMago R et al. (2010) An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat Theoretical and Applied Genetics 122:735-744.
ReferenceSpielmeyer W et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr2 MAS Wheat. Marker Assisted Selection in Wheat.
Type
CAPS
PCR primers
csSr2-F 5'- CAA GGG TTG CTA GGA TTG GAA AAC -3A'
csSr2-R 5'- AGA TAA CTC TTA TGA TCT TAC ATT TTT CTG -3A
Amplification Conditions
Denaturing step: 95C, 2 min. Amplification step (30 cycles): 95C, 30 sec; 60C, 40 sec; 72C, 50 sec. Extension step: 72C, 5 min. 15C, 1 min
Specificity
Detects three different alleles of Sr2: 'null', Marquis, and Hope. See MAS wheat page for more details