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GrainGenes Reference Report: TAG-129-1843

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Reference
TAG-129-1843
Title
Development and validation of KASP assays for genes underpinning key economic traits in bread wheat.
Journal
Theoretical and Applied Genetics
Year
2016
Volume
129
Pages
1843-60
Author
Rasheed A
[ Show all 11 ]
Abstract
We developed and validated a robust marker toolkit for high-throughput and cost-effective screening of a large number of functional genes in wheat. Functional markers (FMs) are the most valuable markers for crop breeding programs, and high-throughput genotyping for FMs could provide an excellent opportunity to effectively practice marker-assisted selection while breeding cultivars. Here we developed and validated kompetitive allele-specific PCR (KASP) assays for genes that underpin economically important traits in bread wheat including adaptability, grain yield, quality, and biotic and abiotic stress resistances. In total, 70 KASP assays either developed in this study or obtained from public databases were validated for reliability in application. The validation of KASP assays were conducted by (a) comparing the assays with available gel-based PCR markers on 23 diverse wheat accessions, (b) validation of the derived allelic information using phenotypes of a panel comprised of 300 diverse cultivars from China and 13 other countries, and (c) additional testing, where possible, of the assays in four segregating populations. All KASP assays being reported were significantly associated with the relevant phenotypes in the cultivars panel and bi-parental populations, thus revealing potential application in wheat breeding programs. The results revealed 45 times superiority of the KASP assays in speed than gel-based PCR markers. KASP has recently emerged as single-plex high-throughput genotyping technology; this is the first report on high-throughput screening of a large number of functional genes in a major crop. Such assays could greatly accelerate the characterization of crossing parents and advanced lines for marker-assisted selection and can complement the inflexible, high-density SNP arrays. Our results offer a robust and reliable molecular marker toolkit that can contribute towards maximizing genetic gains in wheat breeding programs.
External Databases
PubMed: 27306516
Probe
[ Hide all but 1 of 70 ]
Rht-B1_SNP
Rht-D1_SNP
Lr34_TCCIND
Lr34jagger
Sr2_ger9 3p
snp3BS-8
UMN10_SNP
1RS:1AL_8035
1RS:1BL_6110
Glu-D1d_SNP
Sr36/Pm6_8068
GCP_DUP
Pinb-D1_INS
Pina-D1_INS
Tsn1
TaSus2-2B_SNP
WKS
TaPpdDD001
TaPpdDI001
TaPpdDD002
TaPpdBJ001
GS100-1027IND
GS105-1117IND
Psy1Da-g
Vrn-A1_9K0001
Vrn1_new
Vrn-A1b-Marq
Exon7_C/T_Vrn-A1
Vrn-B1_A
Vrn-B1_B
Vrn-D1-D1a_A
Glu-Ax1/x2*_SNP
Glu-Ax2_IND
ubw14
TaGS-D1
PODA1_462_SNP
TaSdr-B1
1fehw3
PPO-A1
PPO-D1_SNP
LoxB1_SNP
Zds-A1_SNP
Vp1B1-193
Vp1B2-83
TaCKX-D1
TaGASR
Rht-B1_197IND
Rht-B1_160IND
PsyB1c
Wx-B1-IND
Pinb2-v2-3
PHS1-646
PHS1-666
Yr15-R5
Yr15-R8
Pds-B1_2002
LYCB1_3765_SNP
CWI_4A_1523
CWI_5D_312
BX13-1510
7OE-866
TGW6-1050-AL1
PsyA1-IND
TaMoc-2433
TaMFT_721J
TaMFT_1617R
Lr47-1
Lr68-2
Lr21 GQ504819-1346-C/T
Dreb-B1

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