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GrainGenes Reference Report: TAG-134-993

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Reference
TAG-134-993
Title
Characterization of wheat curl mite resistance gene Cmc4 in OK05312
Journal
Theoretical and Applied Genetics
Year
2021
Volume
134
Pages
993-1005
Author
Zhao L
Liu S
Abdelsalam NR
Carver BF
Bai G
Abstract
Key message. Cmc4, a wheat curl mite resistance gene, was delimited to a 523 kb region and a diagnostic marker haplotype was identified for selecting Cmc4 in breeding programs. Abstract. Wheat curl mite (WCM, Aceria tosichella Keifer) is a disastrous wheat pest in many wheat-growing regions worldwide. WCM not only directly affects wheat yield, but also transmits wheat streak mosaic virus. Growing WCM resistant cultivars is the most economical and sustainable method to reduce its damage. A hard winter wheat breeding line OK05312 (PI 670019) carries Cmc4 gene resistance to A. tosichella and has many desirable agronomic traits. To finely map Cmc4 in OK05312, two recombinant inbred line populations were developed from crosses between OK05312 and two susceptible cultivars, SD06165 and Jerry, genotyped using single nucleotide polymorphism (SNP) markers generated from genotypingby-sequencing (GBS), and phenotyped for WCM resistance. Gene mapping using the two SNP maps confirmed Cmc4 in OK05312 that explained up to 68% of the phenotypic variation. Further analysis delimited Cmc4 to a ~ 523 kb region between SNPs SDOKSNP6314 and SDOKSNP2805 based on the Ae. tauschii reference genome. We developed 18 polymorphic Kompetitive Allele Specific PCR (KASP) markers using the sequences of GBS-SNPs in this region and 23 additional KASP markers based on the SNPs between the parents derived from 90K SNP chips. The KASP markers SDOKSNP6314 and SDOKSNP9699 are closest to Cmc4 and can be used to diagnose the presence of Cmc4 in wheat breeding programs. Haplotype analysis suggested that CmcTAM112 in TAM112 might be the same gene as Cmc4.
External Databases
https://doi.org/10.1007/s00122-020-03737-3
Probe
[ Hide all but 1 of 23 ]
SDOKSNP7523
SDOKSNP1563
SDOKSNP6314
SDOKSNP2805
SDOKSNP9699
SDOKSNP8
SDOKSNP5470
SDOKSNP102
SDOKSNP2580
SDOKSNP247
SDOKSNP1639
SDOKSNP1714
SDOKSNP755
SDOKSNP1705
SDOKSNP776
370SNP12063
IWA6673_KASP
IWB64081_KASP
IWB27307_KASP
IWB20535_KASP
IWB59282_KASP
IWA5275_KASP
IWB6072_KASP

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