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GrainGenes Reference Report: PLC-14-537

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Reference
PLC-14-537
Title
An integrated physical and genetic map of the rice genome
Journal
Plant Cell
Year
2002
Volume
14
Pages
537-545
Author
Chen MS
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Abstract
Summary: Rice was chosen as a model organism for genome sequencing because of its economic importance, small genome size, and syntenic relationship with other cereal species. We have constructed a bacterial artificial chromosome finger-print-based physical map of the rice genome to facilitate the whole-genome sequencing of rice. Most of the rice genome (approximately 90.6%) was anchored genetically by overgo hybridization, DNA gel blot hybridization, and in silico anchoring. Genome sequencing data also were integrated into the rice physical map. Comparison of the genetic and physical maps reveals that recombination is suppressed severely in centromeric regions as well as on the short arms of chromosomes 4 and 10. This integrated high-resolution physical map of the rice genome will greatly facilitate whole-genome sequencing by helping to identify a minimum tiling path of clones to sequence. Furthermore, the physical map will aid map-based cloning of agronomically important genes and will provide an important tool for the comparative analysis of grass genomes
External Databases
Pubmed: 11910002
Keyword
arms
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