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GrainGenes Reference Report: PMB-49-137

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Reference
PMB-49-137
Title
Do 14-3-3 proteins and plasma membrane H+-ATPases interact in the barley epidermis in response to the barley powdery mildew fungus?
Journal
Plant Molecular Biology
Year
2002
Volume
49
Pages
137-147
Author
Finnie C
[ Show all 8 ]
Abstract
Summary: 14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H(+)-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with the powdery mildew fungus. We have isolated a cDNA encoding a plasma membrane H(+)-ATPase (HvHA1), which is also induced by powdery mildew attack. The C-terminal domain of this H(+)-ATPase interacts with 14-3-3 proteins in the yeast two-hybrid system. Inoculation with the powdery mildew fungus, or treatment with fusicoccin, results in an increase in fusicoccin binding ability of barley leaf membranes. Overlay assays show a fungus-induced increase in binding of digoxygenin-labelled 14-3-3 protein to several proteins including a 100 kDa membrane protein, probably the plasma membrane H(+)-ATPase. These effects are seen specifically in the inoculated epidermis and not in the whole leaf. We propose that 14-3-3 proteins are involved in an epidermis-specific response to the powdery mildew fungus, possibly via an activation of the plasma membrane H(+)-ATPase
External Databases
Pubmed: 11999370
Keyword
14-3-3 protein
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