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GrainGenes Reference Report: TAG-82-537

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Reference
TAG-82-537
Title
Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat.
Journal
Theoretical and Applied Genetics
Year
1991
Volume
82
Pages
537-544
Author
Rogowsky PM
Guidet FLY
Langridge P
Shepherd KW
Koebner RMD
Abstract
The introgression of genetic material from alien species is assuming increased importance in wheat breeding programs. One example is the translocation of the short arm of rye chromosome 1 (1RS) onto homoeologous wheat chromosomes, which confers disease resistance and increased yield on wheat. However, this translocation is also associated with dough quality defects. To break the linkage between the desirable agronomic traits and poor dough quality, recombination has been induced between 1RS and the homoeologous wheat arm 1DS. Seven new recombinants were isolated, with five being similar to those reported earlier and two having a new type of structure. All available recombinants were characterized with DNA probes for the loci Nor-R1, 5SDna-R1, and Tel-R1. Also, the amount of rye chromatin present was quantified with a dispersed rye-specific repetitive DNA sequence in quantitative dot blots. Furthermore, the wheat-rye recombinants were used as a mapping tool to assign two RFLP markers to specific regions on chromosome arms 1DS and 1RS of wheat and rye, respectively.
Germplasm
Chinese Spring T1DL.1RS / 3*Halberd /2/ 3*Warigal
[ Show all 18 ]
Locus
5SDNA
Tri25-11
IPSR161
Nor-R1
Map Data
Wheat.Rye recombinant
Probe
pL10a2
pScR4 TaqI 2.4kb
Rearrangement
WD-1
[ Show all 10 ]
Keyword
Homoeologous recombination
mapping
Repetitive DNA sequences
RFLP markers
Wheat-rye recombinants
Image
1D.1R recombinants