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GrainGenes Reference Report: TAG-83-931

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Reference
TAG-83-931
Title
RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye
Journal
Theoretical and Applied Genetics
Year
1992
Volume
83
Pages
931-939
Author
Devos KM
[ Show all 6 ]
Type
Article
Language
English
Abstract
Genetic maps of chromosomes 3A, 3B and 3D of wheat and 3R of rye were developed using 22 DNA probes and two isozyme marker systems. Analysis of the 49 loci mapped showed extreme clustering around the centromere in all four maps, with large 'gaps' in the distal chromosome regions, which is interpreted as being due to strong localisation of recombination towards the ends of the wheat and rye chromosomes. In the centromeric regions gene orders are highly conserved between the three wheat genomes and the rye genome. However, the unpredictable behaviour of the DNA clones that map in distal chromosome locations may indicate that the genomes are diverging most rapidly in the regions of higher recombination. A comparison of cDNA and genomic probes showed the latter to be much more efficient for revealing RFLP. Some classes of gDNA clones, i.e. chromosome-specific sequences and those hybridizing in a non-homoeologous manner, were seen to be most polymorphic. Correlations between map locations and RFLP levels showed no clear relationship. In addition to anonymous DNA clones, the locations of known function clones, sedoheptulose-1,7-bisphosphatase (XSbp), carboxypeptidase I (XCxp1) and a bZIP protein (XEmbp), were ascertained along with those for two isozyme loci, Mal-1 and Est-5.
Locus
Xpsr598-3A
[ Show all 75 ]
Map Data
Wheat, Gale
Keyword
genetic maps
isozymes
restriction fragment length polymorphism
rye
wheat
Gene Catalogue Number
182
Cited in
ReferenceMcIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.