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GrainGenes Reference Report: TAG-86-705

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Reference
TAG-86-705
Title
A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome
Journal
Theoretical and Applied Genetics
Year
1993
Volume
86
Pages
705-712
Author
Kleinhofs A
[ Show all 22 ]
Type
Article
Language
English
Abstract
A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), 5 isozyme, 2 morphological, 1 disease resistance and 7 specific amplicon polymorphism (SAP) markers. The RFLP identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosome 1, 2 and 3 and the long (minus) are of chromosome 7.
Germplasm
Morex (barley)
Steptoe (barley)
Locus
Hor1
[ Show all 13 ]
Map Data
5HS, Steptoe x Morex
Barley, SxM
Probe
ABG654
[ Show all 13 ]
Keyword
barley
centromeres
genome
mapping
restriction fragment length polymorphism