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GrainGenes Reference Report: TAG-87-789

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Reference
TAG-87-789
Title
Evaluation of 'sequence-tagged-site' PCR products as molecular markers in wheat
Journal
Theoretical and Applied Genetics
Year
1994
Volume
87
Pages
789-794
Author
Talbert L
Blake N
Chee P
Blake T
Magyar G
Abstract
The polymerase chain reaction PCR) is an attractive technique for many genome mapping and characterization projects One PCR approach which has been evaluated involves the use of randomly amplified polymorphic DNA (RAPD) An alternative to RAPDs is the sequence-tagged-site (STS) approach, whereby PCR primers are designed from mapped lowcopy-number sequences In this study, we sequenced and designed primers from 22 wheat RFLP clones in addition to testing 15 primer sets that had been previously used to amplify DNA sequences in the barley genome Our results indicated that most of the primers amplified sequences that mapped to the expected chromosomes in wheat. Additionally, 9 of 16 primer sets tested revealed polymorphisms among 20 hexaploid wheat genotypes when PCR products were digested with restriction enzymes. These results suggest that the STS-based PCR analysis will be useful for generation of informative molecular markers in hexaploid wheat.
Keyword
dna
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