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GrainGenes Reference Report: TAG-102-1273

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Reference
TAG-102-1273
Title
Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers
Journal
Theoretical and Applied Genetics
Year
2001
Volume
102
Pages
1273-1279
Author
Masojc P
Myskow B
Milczarski P
Abstract
Summary: RFLP-based genetic map of rye, developed previously using a cross of lines DS2xRXL10 (F2 generation), was extended with 69 RAPD and 12 isozyme markers. The actual map contains 282 markers dispersed on all seven chromosomes and spans a distance of 1,140 cM. The efficiency of mapping RAPD markers was close to ten loci per 100-screened arbitrary primers. A strong selection of polymorphic, intensive and reproducible fragments was necessary to reveal individual marker loci that could be assigned to rye chromosomes. Newly mapped markers cover a substantial part of the rye genome and constitute a valuable tool suitable for map saturation, marker-aided selection and phenetic studies. A specific nomenclature for the RAPD loci mapped on individual rye chromosomes, which could be helpful in managing of accumulating data, is proposed
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/1102008/11021273.htm
Gene
Sod-R2 (Secale)
[ Show all 11 ]
Map Data
Rye, Ds2 x RxL10, RAPD
Probe
APR1.5
[ Show all 69 ]
Keyword
[ Hide all but 1 of 39 ]
amplified polymorphic dna
arbitrary primers
chromosome
cross
crosses
distance
dna
fragments
generation
genetic
genetic map
genome
isozyme
isozyme markers
line
linkage map
loci
map
mapping
marker
marker loci
marker-aided selection
nomenclature
p
pcr
plant science
polymorphic
polymorphic dna
primer
random amplified polymorphic dna
rapd
rapd markers
rflp
rye
rye chromosomes
secale cereale
selection
span
tool